Title: Traction Force Microscopy on Polyacrylamide Hydrogels
Adherent cells can both sense and generate forces in their environment through linkages to the extracellular matrix. These mechanical forces between cells and the extracellular matrix evolve during growth, development, and change with disease states. In this talk, I will review Traction Force Microscopy methods using polyacrylamide hydrogels to modulate the mechanical environment while simultaneously measuring cell-generated forces. I will also discuss characterization of polyacrylamide materials applied to mechanobiology studies and compare Traction Force Microscopy platforms to micropillar arrays for measuring cell-generated forces.